Development of a novel continuous Extracellular Vesicle production system

Acronym: greenEV 

GreenEV is an interdisciplinary project funded by the Horizon 2020 Marie Skłodowska-Curie Action Individual Fellowship (MSCA-IF) to build bridges between EV research, plant biology, biotechnology, and food nanotechnology. MSCA fellow Maneea Moubarak from Damanhour University, Egypt will develop a novel continuous plant EVs production platform at EVs & MS laboratory. 

Plant cells similar to mammalian cells secrete phospholipid membrane-surrounded vesicles. In the greenEV project, we exploit plant cell suspension cultures of tomato (Solanum Lycopersicum L) and gingko (Ginkgo biloba L) for the production of EVs. There are many practical reasons for choosing tomato and gingko for our studies, such as both species have sequenced genomes that make feasible their molecular studies. Moreover, tomato is a nutritious, healthy, and high economic value food, and one of the symbols of Italian-made agri-food products worldwide. Ginkgo is a member of a very old genus, with some fossils dating back 270 million years. Leaves and seeds of ginkgo have various uses in traditional herbal medicine and modern supplement formulations. Gingko biloba has high heterozygosity and it is an endangered plant in Egypt. The specific research objectives of greenEV are: 

  • Set up a new continuous ginkgo and tomato cell-based EV production system 
  • Characterization of gingko and tomato cell-derived EVs using an integrated pipeline 
  • Transformation of the EVs into nutraceutical and drug delivery system to overcome on limitations of current EV therapeutics 

None of these objectives has been addressed before and they have high potential to expand the knowledge in the field and to drive the research activity towards industrialized continuous production of plant EVs. The research objectives are integrated with concerted training objectives in EV research, omics, outreach program, dissemination events, and considerable knowledge transfer in plant tissue culturing from the researcher to the IBBR-CNR host group. 

The beneficiary of the grant is the National Research Council of Italy. The scientific responsible for the project is Gabriella Pocsfalvi. Other partners are Prof. Pasquale Chiaiese from the Department of Agricultural Sciences, University of Federico II, Italy, Prof. Veronika Krlaj-Iglič from the Faculty of Health Sciences, University of Ljubljana, Slovenia and Prof. Jozsef Jeko from the Agricultural and Molecular Research, University of Nyíregyháza, Hungary

Link: https://cordis.europa.eu/project/id/895579/it 

This project has received funding from the FET Proactive programme under grant agreement Nº 895579 

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EVs & MS laboratory

Institute of Biosciences and BioResources

National Research Council

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